USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. It’s involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. It binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Source:Recombinant corresponding to aa2-858 from human USP5, fused to FLAG-tag at N-terminal, expressedin Sf9 insect cells via a baculovirus expression system.
Molecular Weight:~96kD
Applications:Suitable for for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Source Antigen：Recombinant, Sf9 insect cells
Grade：Purified
Purity：Purified (~55%)
Form：Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 10% glycerol, 3mM DTT, 90ug/ml FLAG peptide.
