UCH-L3 (Ubiquitin carboxyterminal hydrolase isozyme 3) is a 26-28kD member of the peptidase C12 family of enzymes. It shows wide expression, being noted in adipocytes, renal duct epithelium, neurons, and striated muscle. UCH-L3 cleaves both monomeric ubiquitin (Ub) from Ub-protein conjugates, and a GGLRQ peptide from the C-terminus of the Ub-like protein NEDD-8. Notably, UCH-L3 activity is muted in the presence of Ub interacting dimers. Human UCH-L3 is 230aa in length. It is phosphorylated on Ser75 and Ser130, and contains two ubiquitin-binding sequences between aa40-57 and 178-186. Four potential splice forms have been reported. Two show a four aa deletion between aa15-18, with one also containing a 14aa substitution for aa179-230. Two others contain an alternative start site at Met37, one of which is also accompanied by a seven aa substitution for aa143-230. Full-length human UCH-L3 shares 98% aa identity with mouse and rat UCH-L3.
Source:Recombinant corresponding to aa2-231 from human UCHL3, fused to His-tag at N-terminal, expressed in E. coli.
Molecular Weight:~27.1kD
Applications:Suitable for for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 225mM imidazole, 10% glycerol, 3mM DTT.
