Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4D is a regulator of airway smooth-muscle contractility, and has been identified as a potential risk predictor for ischemic stroke. Additionally, PDE4D has been associated with asthma pathophysiology and bone formation. The PDE4D gene encodes at least 9 different isoforms.
The PDE4D inhibitor screening assay kit comes in a convenient 96-well format, with purified PDE4D3 enzyme, fluorescently labeled PDE4D substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE4D Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4D reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4D for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.
Intended Use:Designed for identification of inhibitors of PDE4D3 using fluorescence polarization.
Test Principle:The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4D to the binding agent.
Storage and Stability:Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
