Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE3B, also known as cGMP-inhibited phosphodiesterase, is involved in mediating the antilipolytic and antiglycogenlytic effects of insulin in adipose and liver tissues. The PDE3B Assay Kit is designed for identification of PDE3B inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE3B to the binding agent.
Intended Use:Designed for identification of PDE3B inhibitors using fluorescence polarization.
Test Principle:The PDE3B inhibitor screening assay kit comes in a convenient 96-well format, with purified PDE3B enzyme, fluorescently labeled PDE3 substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE3B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE3B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE3B for 1 hour. Second, binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.
Storage and Stability:Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
