PDE1B is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.
Source:Recombinant corresponding to full length rat PDE1B, fused to His-tag at N-terminal, expressed in Sf9 insect cells via a baculovirus expression system.
Molecular Weight:~89kD
Specific Activity:~3600pmol/min/ug
Unit Definition:One unit is defined as the amount of enzyme that will convert 1pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C.
Applications:Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, Sf9 insect cells
Grade：Purified
Purity：Purified (~70%)
Form：Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 200mM Imidazole, 3mM DTT, 20% glycerol.
