Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE1A1 is believed to regulate vascular smooth muscle contraction and may play a role in sperm function.
Intended Use:The PDE1A BioAssay™ Kit is designed for identification of PDE1A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE1A1 to the binding agent.
Test Principle:The PDE1A inhibitor screening BioAssay™ kit comes in a convenient 96-well format, including purified PDE1A1 enzyme, fluorescently labeled PDE1A substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE1A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE1A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE1A1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.
Materials or Instruments Required But Not Supplied:Fluorescent microplate reader capable of measuring fluorescence polarization
Storage and Stability:Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
