The PCR Cloning Kit is a simple and highly efficient method to insert any gene or DNA fragment into a vector, without time-consuming ligation steps.
Test Principle:A primer set homologous to the gene of interest and the vector is used to amplify a PCR product. This purified PCR product is incubated with restriction enzyme-digested vector and Reagent A of the Quick PCR cloning kit. Following a brief incubation with Reagent B, the annealed vector is ready for transformation.
Kit Components:Reagent AReagent BBuffer ABSA10X (0.1% w/v)distilled water
Storage and Stability:Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
