Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Source:Recombinant corresponding to aa2-104 from full length human Histone 4, fused to His tag at N-terminal, expressed in E. coli.
Molecular Weight:~12.1kD
Applications:Suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥93% (SDS-PAGE)
Form：Supplied as a liquid in 8mM PBS, pH 7.4, 110mM sodium chloride, 2.2mM potassium chloride, 3mM DTT, 20% glycerol.
