Histones are small basic proteins which constitute the genome of eukaryotes. The chromosomes of eukaryotes contain five major types of histones, H1, H2A, H2B, H3 and H4. Two each of the class H2A, H2B, H3 and H4, designated as core histones, assemble to form one octameric nucleosome core particle by wrapping 146 base pairs of DNA, while the linker histone H1 binds the nucleosome and the entry and exit sites of the DNA, thus locking the DNA into place. The number of histone H3 variants differs among species. In mammals, they comprise four main members: H3.1 and H3.2 are the replicative histones expressed during S phase; H3.3 is the replacement histone expressed throughout the cell cycle; and centromeric protein A (CENP-A) is specifically present at centromeres. The unique mammalian H3 variant, H3.1, play a specialized role in chromatin biology that may correlate with differentiation or cell origin determination. Human H3.1 is localized on chromosomal region 6p21.3.
Source:Recombinant corresponding to aa2-58 from human Histone 3, fused to GST tag at N-terminal, expressed in E. coli.
Molecular Weight:~32kD
Applications:Suitable for use as a substrate for histone methyltransferases. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：Purified (~70%)
Form：Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 20% glycerol, 3mM DTT.
