CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation.
Cyclin-A2 (CCNA2) is a member of the Cyclin family which act as regulators of CDK kinases. CCNA2 is essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
Source:Recombinant corresponding to aa1-298 from human CDK2 and aa174-432 from human CyclinA2 fused to His-GST tags at N-terminal, coexpressed in a baculovirus Sf9 insect cell expression system.
Molecular Weight:CDK2: ~61kD; CyclinA2: ~75kD
Applications:Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Specific Activity:~390pmol/min/ug
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Sf9 insect cells
Grade：Purified
Purity：Purified (~64%)
Form：Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 16mM glutathione, 2.2mM potassium chloride, 20% glycerol, 3mM DTT.
