The c-ABL proto-oncogene encodes a protein tyrosine kinase that is located in the cytoplasm and nucleus. In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, the c-ABL proto- oncogene undergoes a chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) as a result of the fustion of c-ABL sequences from chromosome 9 to the Bcr gene on chromosome 22. The molecular consequence of this translocation is the generation of a chimeric Bcr/ABL mRNA encoding activated ABL protein tyrosine kinase.
Source:Recombinant corresponding to aa248-531 from human Abelson murine leukemia viral oncogene homolog 1, with T334I mutation, fused to His-tag at N-terminal, expressed in a Baculovirus-infected Sf9 cell expression system.
Molecular Weight:~33kD
Applications:Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Specific Activity:~222pmol/min/ug
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, Sf9 cell
Grade：Purified
Purity：Purified (~75%)
Form：Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween 20, 20% glycerol, 3mM DTT.
