SPA17 is a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis.
Recombinant protein corresponding to aa1-151human SPA17, fused to His-tag at N-terminal expressed in E. coli. (NP_059121)
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E.coli
Grade：Purified
Purity：~90% (SDS-PAGE, reducing conditions and visualized by coomassie blue stain)
Form：Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol
