Transforming growth factor betas (TGF Betas) mediate many cell-cell interactions that occur during embryonic development. Three TGF Betas have been identified in mammals. TGF Beta 1, TGF Beta 2 and TGF Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
TGF-b3 Mouse Recombinant produced in E. coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 112 amino acids and having a molecular mass of 25.5kD.
Biological Activity:The activity is determined by the ability to induce chondrogenic differentiation.
Protein Content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics)
2. Analysis by RP-HPLC, using a calibrated solution of TGF-b 3 as a Reference Standard.
Storage and Stability:May be stored at 4°C for short-term only. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：E. coli
Grade：Highly Purified
Purity：~96% (SDS-PAGE)
Form：Supplied as a liquid in 20% ethanol, 0.12% acetic acid.
