Zinc-alpha-2-glycoprotein (ZAG) is found in body fluids such as serum, sweat, and seminal and breast cyst fluids. It is identical in aa sequence to tumor-derived lipid mobilizing factor (LMF), a protein associated with the dramatic loss of adipose body stores in cancer cachexia, and has been shown to stimulate lipolysis by adipocytes in vivo and in vitro. A role for ZAG has been proposed in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and also it regulates melanin production by normal and malignant melanocytes. It has also recently been classified as a novel adipokine in that it is produced by both white and brown fat adipocytes and may be useful for the study of Diabetes and Obesity.
ZA2G is a single, glycosylated polypeptide chain containing a total of 290 amino acids encoding (13-290).
Recombinant protein corresponding to human ZA2G with 12 extra amino acids fused at the N-terminus expressed in HEK cells. Recombinant protein is identical to Swiss-Prot-P25311 (aa18-295, mature Zinc-Alpha-2-Glycoprotein).
Biological Activity:Differentiated human SGBS adipocytes were incubated for 18h at two dose levels of rhZA2G - 5 and 20ug/ml. Lipolysis was quantified by measuring glycerol release into the medium using a standard protocol. Isoproterenol (10uM) and IBMX (100uM) were used as positive controls. "Con" stands for the negative control. There was a 3-fold increase in glycerol release with both doses. The increase was statistically significant at 5 µg/ml dose of rhZA2G (p<0.01) as well as in positive controls.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：2ug
Source Antigen：293 cells (HEK)
Grade：Highly Purified
Purity：~90% (RP-HPLC and SDS-PAGE)
Form：Supplied as a lyophilzed powder with no additives. Reconstotute with sterile ddH2O to ≥0.1mg/ml.
