GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Source:Recombinant corresponding to human GDNF, expressed in E. coli.
Molecular Weight:~30.36kD
Biological Activity:hGDNF is fully biologically active when compared to a standard. The ED50, calculated by the dose-dependent dopamine uptake in rat mesencephalic cultures, was found to be 4-8ng/ml.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：2ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (RP-HPLC and SDS-PAGE)
Form：Supplied as a lyophilized powder from 10mM sodium citrate, 100mM sodium chloride, pH 4.0. Reconstitute with sterile ddH2O to 100ug/ml.
