p21/WAF1/Cip1/Sdi1/Pic1 is a tumor suppressor protein. Expression of p21/WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21/WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. Meanwhile, the p21/WAF1 protein also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process. Evidently, inactivation of p53 disrupts the p53-dependent expression of p21/WAF1. p21/WAF1is a tumor suppressor involved in the pathogenesis of a variety of malignancies.
Source:Recombinant corresponding to human CDKN1A, expressed in E.coli.
Molecular Weight:~47kD
Endotoxin: ~0.1ng/ug. Diners and Aggregates: <1% as determined by coomassie blue SDS-PAGE gel analysis.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：2ug
Source Antigen：Recombinant, E.coli
Grade：Highly Purified
Purity：~90% (HPLC and SDS-PAGE)
Form：Supplied as a liquid in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% gycerol. No preservative added.
