RGS1 is a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120aa motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal.
Source:Recombinant corresponding to aa1-209 from human RGS1, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight:~26kD (233aa) confirmed by MALDI-TOF
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E.coli
Grade：Highly Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.15M sodium chloride, 10% glycerol.
