TNF-R1 is a receptor for TNF-alpha and homotrimeric lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.
Biological Activity:TNF-R1:Fc fusion protein blocks TNF-alpha-mediated biological effect with an inhibitory activity in a concentration range of 0.1 to 1ug/ml.
Sequence:The extracellular domain of human TNF-R1 (aa 22-211) is fused to the Fc portion of human IgG1.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
