Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction.
Recombinant protein corresponding to aa85-223 with a FLAG®-tag at the N-Terminus of human, expressed in E. coli
Biological Activity:Induces cell death at a concentration range of 0.1-1ng/ml (ED50=0.05ng/ml or 2x10e7 units/mg; WEHI 164 cells).
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：E. coli
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a lyophilized powder from PBS pH 7.2. rReconstitute with 100ul sterile ddH2O.
