Periostin was originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta1 (TGF-beta1) and bone morphogenetic protein (BMP-2). Abnormal expression of periostin is also linked to angiogenesis and metastasis in epithelial tumors.
Sequence:Mouse periostin (aa 24-783) (isoform 5) is fused at the C-terminus to a FLAG®-tag, expressed in CHO cells.
Molecular Weight:~85kD (SDS-PAGE)
Biological Activity:Rescues sphere formation from periostin KO primary tumor cells at 50ng/ml.
Endotoxin:<0.1EU/ug (LAL test; Lonza)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Mouse, CHO cells
Grade：Highly Purified
Purity：>95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS. Reconstitute with 100ul sterile ddH2O.
