OX40L is a membrane-expressed cytokine that belongs to the TNF ligand family. It acts as co-stimulator through interaction with its ligand OX40 on T cells, stimulating T cell activation, proliferation and cytokine production. OX40L is expressed on antigen presenting cells including B cells, dendritic cells, mast cells and endothelium.
This hexameric protein is a high activity construct in which two trimeric OX40L ligands are artificially linked via the collagen domain of ACRP30 and therefore mimics the membrane-bound form of the protein. This construct very effectively activates T cell proliferation.
Biological Activity:Activates T cell proliferation.
Sequence:Each monomer in this construct consists of the extracellular domain of mouse OX40L (aa 52-199) fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：HEK 293 cells
Grade：Highly Purified
Purity：≥90% (SDS-PAGE). Endotoxin: ≤0.1EU/ug protein (LALmethod).
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS. Reconstitute with 100ul sterile dH2O.
