IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.
Recombinant protein corresponding to mouse IDO (aa1-407), at C-terminus fused to His-tag.
Biological Activity:Specific Activity: >100,000U/mg protein with L-tryptophan as substrate. One unit is defined as the amount of enzyme that produces 1nmol of kynurenine per hour.
Heme Content:Not determined for end product. 7uM hemin was adde for protein induction.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：E. coli
Grade：Highly Purified
Purity：>90% (SDS-PAGE) Endotoxin: ≤1EU/ug (LAL)
Form：Supplied as a 0.2um filtered liquid in 50mM TRIS-Cl, pH 7.4, 1mM EDTA.
