FGF-23 (Fibroblast growth factor 23) is a regulator of phosphate homeostasis. It upregulates EGR1 expression in the presence of KLBy. Acts directly on the parathyroid to decrease PTH secretion. Regulates the vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF-23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) and of hyperphosphatemic familial tumoral calcinosis (HFTC).
Sequence:Signal peptide and human FGF-23 (aa1-251) are fused at the C-terminus to a His-tag, expressed in HEK 293 cells.
Biological Activity:Activates ERK and FRS2α phosphorylation in Klotho expressing cells.
Molecular Weight:~30kD (SDS-PAGE, full-length). FGF23 is cleaved between Arg179 and Ser180 (two bands of ~14kD and ~16kD).
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
