FasL is a cytokine that binds to TNFRSF6/Fas, a receptor that transduces the apoptotic signal into cells. Is involved in cytotoxic T cell mediated apoptosis and in T cell development.
Sequence:The extracellular domain of human FasL (aa103-281) is fused at the N-terminus to a FLAG®-tag.
Molecular Weight:~33kD (SDS-PAGE)
Endotoxin:<0.05EU/ug (LAL)
Biological Activity:Induces apoptosis of human Jurkat T cells at a concentration of <1ng/ml in the presence of 0.1-1ug/ml TNF Ligands Enhancer. In the absence of TNF Ligands Enhancer, FasL is working at 50-100 fold higher concentrations.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, HEK 293 cells
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS. Reconstitute with 100ul sterile ddH2O.
