DLK1, Soluble (human) BioAssay™ ELISA Kit (Protein Delta Homolog 1, pG2, Fetal Antigen 1, FA 1) is a sandwich ELISA used for the in vitro quantitative determination of human DLK1 in serum and cell culture supernatant.
Expected Values:DLK1 levels range in human serum from 0.4 to >2.5ng/ml (healthy donors)
Assay Range:0.469-30ng/ml
Sensitivity (Limit of Detection):336pg/ml
Intra-assay CV %: ≤10%
Inter-assay CV %: ≤10%
Specificity:This ELISA is specific for the measurement of natural and recombinant human DLK1. It does not cross-react with human DLL1, human DNER, human adiponectin, human progranulin, human ANGPTL3, human RBP4, human resistin, human vaspin, human GPX3, human Nampt, human IL- 33, human clusterin, human leptin, human CTRP5, mouse Nampt, rat Nampt.
Assay Principle:This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human DLK1 in biological fluids. A monoclonal antibody specific for DLK1 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, DLK1 is recognized by the addition of a purified polyclonal antibody specific for DLK1 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-IgG (HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction ismeasured at 450nm after acidification and is directly proportional to DLK1 in the samples.
Storage and Stability:Store *044738F powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
