CD40 belongs to the TNF-receptor superfamily and is essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development and germinal center formation. The interaction of CD40-CD40L is necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells and macrophages. Consistent with its widespread expression on normal cells, CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.
Sequence:The Fc portion of human IgG1 is fused to the C-terminus of human CD40 (aa 21-193), expressed in HEK 293 cells
Binds human and mouse CD40L.
Biological Activity:Blocks the binding of CD40L to CD40.
Molecular Weight:~54kD (SDS-PAGE)
Endotoxin:<0.01EU/ug (LAL test; Lonza).
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, Human, HEK 293 cells
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, pH 7.2. Reconstitute with 50ul sterile ddH2O. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
