Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the 'calreticulin/calnexin cycle' that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule. Recently, Calreticulin was shown to enhance the merger of macrophages and tumor cells, increasing phagocytosis.
Sequence:Human calreticulin (aa18-417) is fused at the C-terminus to a His-tag, expressed in E. coli.
Molecular Weight:~55kD (SDS-PAGE)
Endotoxin:<1EU/ug (LAL test; Lonza).
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Human, E. coli
Grade：Highly Purified
Purity：≥90% (SDS-PAGE)
Form：Supplied as a liquid from a 0.2um-filtered solution in 55mM TRIS-Cl, pH 8.2, 150mM NaCl.
