The Neurofilament H, phosphorylated (NF-H, NFH, NEFH) BioAssay™ ELISA Kit measures the levels of phosphorylated NF-H in human, bovine, chicken, equine, mouse and rat serum, plasma and cerebrospinal fluid. Quality control pNF-H is purified from bovine spinal cord. This ELISA assay is intended for research use only.
Principle of the Assay:This ELISA Kit provides a convenient method for measuring level of protein targets within a single, small-volume sample. Neurofilament H, phosphorylated (NF-H, NFH, NEFH) BioAssay™ ELISA Kit is a 2-site sandwich enzyme linked immunoassay providing a plate pre-coated with mouse monoclonal capture antibody against pNF-H. The end-user incubates the samples on the plate and any pNF-H protein in the sample binds to the capture antibody. The end-user then incubates with a solution containing another mouse monoclonal detection antibody conjugated with HRP, which binds to the captured pNF-H. The end-user adds a developer solution that produces a colored product in wells containing HRP, and the intensity of the color corresponds to the amount of pNF-H in the sample. Finally, the end-user stops the reaction by adding H2SO4. The color changes from blue to yellow and can be quantified by measurement on a suitable ELISA plate reader using an absorbance wave length of 450nm.
Storage and Stability:Store *031402B and *031402C at -20°C. Store all other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
