Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model with human ApoA5 produced 50–70% lower concentrations of mouse serum triglycerides. In addition, peroxisome proliferator-activated receptor- agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Despite these compelling molecular biology data, relatively little is known about ApoA5 protein in human serum
This antibody pair detected recombinant apoa5 protein in sandwich ELISA format and could be potential reagents for the development of clinical diagnostic kits.
Applications:Western Bloting: 1:500-1:2000
Immunofluorescence: 1:200-1:1000
ELISA: Suggested dilution 1:10000
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Grade：Purified
Purity：Purified
Form：Purified antibody in PBS, 0.03% sodium azide.
Specificity：Human
Clone # USB：2G1H11, 1F1E8
Isotype：IgG1
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Purified recombinant fragment of human Apoa5 expressed in E. coli.
