Bafilomycin D shares the same mode of action as bafilomycin A1 which has been the analog of choice in cell biology studies of the role of ATPase. Bafilomycin D contains the ring opened side chain and is a much more stable analog of bafilomycin A1. In-house data demonstrate that bafilomycin D is also a much more selective inhibitor of mammalian cells than bafilomycin A which exhibits a broad profile against tumor cells, fungi and nematodes. Limited availability has restricted a more in depth investigation of this metabolite.
Source:Streptomyces sp. MST-AS5343
Molecular Formula:C₃₅H₅₆O₈
Molecular Weight:604.8
Solubility:Soluble in ethanol, methanol, DMF or DMSO.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ethanol, methanol, DMF or DMSO. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
