85-1770-21　DUSP3, Recombinant, Human, aa1-185, His-Tag (Dual Specificity Protein Phosphatase 3, Dual Specificity Protein Phosphatase VHR, Vaccinia H1-related Phosphatase, VHR) 100ug　D9840-06M

特徴

• DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DUSP3 is expressed in both breast and ovarian tissues. DUSP3 Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.
• Source:Recombinant corresponding to aa1-185 from human DUSP, fused to His-tag at N-terminal expressed in E.coli.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP
• Molecular Weight:~22.6kD (MALDI-TOF)
• Biological Activity:Determined by measuring the amount of enzyme hydrolyzing 1nm of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH 7.5 using 10mM of substrate, corresponding to a specific activity of >1750IU/mg.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.