p53 is expressed in a wide variety of tissues and plays a major role in preventing tumor development. It is involved in cell cycle regulation, functioning as a trans-activator to negatively regulate cell division by controlling a set of genes required for this process. It acts as a tumor suppressor in many tumor types by stimulating either apoptosis or growth arrest depending on the physiological circumstances and the cell type. Defects in p53 expression have been implicated in several diseases including; choroid plexus papilloma, lung cancer, head/neck squamous cell carcinomas, oesophageal squamous cell carcinoma, Li-Fraumeni syndrome, and hereditary adrenocortical carcinoma.
Source:Recombinant corresponding to full length human p53, N-terminal c-Myc, 6His-tagged expressed in baculovirus in Sf21 insect cells.
Molecular Weight:~49kD
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, Sf21
Grade：Highly Purified
Purity：≥94% (SDS-PAGE and Coomassie blue staining)
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 0.3M sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
