Intracellular signals mediated by receptor tyrosine kinases play a pivotal role in morphogenesis, cell fate determination and pathogenesis. c-Cbl is a ubiquitin ligase (E3) that facilitates the regulation of these pathways, attenuating signalling through ubiquitination and subsequent protein degradation. c-Cbl contains several functional domains, including a tyrosine kinase-binding domain that associates with a number of protein tyrosine kinases in a phosphorylation-dependent manner, a catalytic RING-finger domain that interacts with E2s and is essential for E3 activity and a C-terminal proline rich domain which mediates interactions with SH3 containing proteins. Mutations in the gene for c-Cbl have been implicated in a number of human cancers, particularly acute myeloid leukaemia.
Recombinant protein corresponding to full length human c-Cbl, fused to GST-tag at N-terminal, expressed by baculovirus in Sf21 insect cells
Molecular Weight:~127kD
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, Sf21
Grade：Purified
Purity：≥75% (SDS-PAGE, Coomassie blue staining)
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
