The Chicken Ghrelin (GHRL) ELISA Kit is a competitive inhibition immunoassay for in vitro quantitative measurement of chicken GHRL in serum, plasma, tissue homogenates and other biological fluids.
Test Principle:This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for chicken GHRL has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled chicken GHRL and unlabeled chicken GHRL (standards or samples) with the pre-coated antibody specific for chicken GHRL. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of GHRL in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of GHRL in the sample.
Detection Range:123.5-10,000pg/ml
Sensitivity:42.6pg/ml
Kit Components:*025271A: Microtiter Plate, 96-well strip plate. Pre-coated, ready to use*025271B: Standard, 2x1vial 025271C: Standard Diluent, 1x20ml*025271D: Detection Reagent A (green), 1x120ul *025271E: Detection Reagent B (red), 1x120ul 025271F: Assay Diluent A, 2x, 1x6ml025271G: Assay Diluent B, 2x, 1x6ml025271H: TMB Substrate, 1x9ml025271K: Stop Solution, 1x6ml025271L: Wash Buffer, 30X, 1x20ml
Storage and Stability:Store *025271A, *025271B, *025271D and *025271E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap
