Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) BioAssay™ ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CYR61 in mouse serum, plasma, tissue homogenates and other biological fluids.
Notes on 024531J Positive Control:Intended Use:The control vials are from mouse serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics:The control vials contain mouse CYR61 in mouse serum (lyophilized powder). Note: The mouse based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.
Expected Values:183.6pg/mlThe concentration of the control was determined by subtracting two standard deviations to the mean optical density value of twenty QC sample replicates and calculating the corresponding concentration.
Usage:Disslove with 150ul standard dilutent and let stand for 10min. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Storage and Stability:Store *024531A, *024531B, *024531D, 024531E and *024531J at -20°C upon receipt. Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C
3. Add 100ul prepared Detection Reagent A immediately. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 5. Aspirate and wash 5 times
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C. 7. Add 50ul Stop Solution. Read at 450nm immediately.
