Cholesterol-25-Hydroxylase (CH25H) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CH25H in human serum, plasma, tissue homogenates and other biological fluids.
Detection Range:0.625-40ng/ml
Sensitivity:0.226ng/ml
Cholesterol-25-Hydroxylase (CH25H) is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
The deduced 272-amino acid peptide has characteristics of a polytopic membrane protein and contains clusters of histidine residues essential for catalytic activity. Northern blot analysis showed very low levels of cholesterol 25-hydroxylase mRNA in 16 different tissues. Immunohistochemistry detected cholesterol 25-hydroxylase in the endoplasmic reticulum and a perinuclear compartment of transfected cells.
Storage and Stability:Desiccation recommended. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt. Store all other reagents at 4°C. Stable for 6 months after receipt of order.
