Peroxisome Proliferator Activated Receptor gamma Coactivator 1 alpha (PPARgC1a) BioAssay™ ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of PPARgC1a in rat tissue homogenates, cell lysates and other biological fluids.
Detection Range:0.312-20ng/ml
Sensitivity:0.125ng/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*027387A: Microtiter Strips, 1x48 wells*027387B: Standard, 2x1 vial027387C: Standard Diluent, 1x20ml*027387D: Detection Reagent A (green), 1x120ul*027387E: Detection Reagent B (red), 1x120ul027387F: Assay Diluent A, 1x12ml027387G: Assay Diluent B, 1x12ml027387H: TMB Substrate, 1x9ml027387K: Stop Solution, 1x6ml027387L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (027387K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *027387A, *027387B, *027387D and *027387E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
