The Ephrin B2 (EFNB2) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EFNB2 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range:0.156-10ng/ml
Sensitivity:0.056ng/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*024841A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use*024841B: Standard, 2x1 vial024841C: Standard Diluent, 1x20ml*024841D: Detection Reagent A (green), 1x120ul*024841E: Detection Reagent B (red), 1x120ul024841F: Assay Diluent A, 1x12ml024841G: Assay Diluent B, 1x12ml024841H: TMB Substrate, 1x9ml024841J: Positive Control, 0.58ng/ml, 1x1vial (Lyophilized)024841K: Stop Solution, 1x6ml024841L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (024841K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *024841A, *024841B, *024841D, *024841E and *024841J at -20°C. After reconstitution of *024841J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 024841J - Positive Control:Intended Use:The control vials are from a human MCF-7 cell lysates sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics:The control vials contain human EFNB2 in human MCF-7 cell lysates sample (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.
Expected Value:0.58ng/ml
Usage:Dissolve with 150ul standard diluent and let stand for 10 minutes. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary:1. Prepare all reagents, samples and standards;2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
