Collagen Type I Alpha 1 (COL1a1) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL1a1 in human serum, plasma and other biological fluids.
Detection Range:0.312-20pg/ml
Sensitivity:0.127pg/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*024263A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use*024263B: Standard, 2x1 vial024263C: Standard Diluent, 1x20ml*024263D: Detection Reagent A green, 1x120ul*024263E: Detection Reagent B (red), 1x120ul024263F: Assay Diluent A, 1x12ml024263G: Assay Diluent B, 1x12ml024263H: TMB Substrate, 1x9ml024263K: Stop Solution, 1x6ml024263L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (024263K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *024263A, *024263B, *024263D and *024263E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
