85-1745-31　Pentosidine (PTD) BioAssay™ ELISA Kit 96Tests　027322

特徴

• The Pentosidine (PTD) ELISA Kit is a competitive inhibition immunoassay for in vitro quantitative measurement of PTD in serum, plasma and other biological fluids.
• Detection Range:4.94-400ng/ml
• Sensitivity:2.28ng/ml
• Intra-Assay CV:<10%
• Inter-Assay CV:<12%
• Kit Components:*027322A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use*027322B: Standard, 2x1vial 027322C: Standard Diluent, 1x20ml*027322D: Detection Reagent A, 1x120ul *027322E: Detection Reagent B, 1x120ul 027322F: Assay Diluent A, 2X, 1x12ml027322G: Assay Diluent B, 2X, 1x12ml027322H: TMB Substrate, 1x9ml027322K: Stop Solution, 1x6ml027322L: Wash Buffer, 30X, 1x20ml
• Storage and Stability:Store *027322A, *027322B, *027322D and *027322E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Procedure Summary:1. Prepare all reagents, samples and standards
• 2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
• 3. Aspirate and wash 3 times
• 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
• 5. Aspirate and wash 5 times
• 6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C
• 7. Add 50ul Stop Solution. Read at 450nm immediately.