The Mouse Proopiomelanocortin (POMC) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of POMC in mouse serum, plasma and other biological fluids.
Detection Range:123.5-10,000pg/ml
Sensitivity:43.1pg/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*027659A: Microtiter Plate, 96 wells, Pre-coated, ready to use*027659B: Standard, 2x1vial 027659C: Standard Diluent, 1x20ml*027659D: Detection Reagent A (green), 1x1vial*027659E: Detection Reagent B (red), 1x120ul 027659F: Assay Diluent A, 1x12ml027659G: Assay Diluent B, 1x12ml027659H: TMB Substrate, 1x9ml027659J: Positive Control, 1462.2pg/ml, 1x1 vial027659K: Stop Solution, 1x6ml027659L: Wash Buffer, 30X, 1x20ml027659M: Detection Reagent A Diluent, 1x300ul
Precaution:The Stop Solution (027659K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *027659A, *027659B, *027659D, *027659E and *027659J at -20°C. After reconstitution of *027659J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 027659J - Positive Control:Intended Use:The control vials are from a mouse serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics:The control vials contain mouse POMC in mouse serum (lyophilized powder). Note: The mouse based serum used in this product are from healthy animal(s) of SPF standard (specific pathogen-free animals).
Expected Value:1462.2pg/ml
Usage:Dissolve with 150ul standard diluent and let stand for 10 minutes. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary:1. Prepare all reagents, samples and standards;2. Add 50ul standard or sample to each well
3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
