85-1744-50　Ficolin 1 (FCN1) BioAssay™ ELISA Kit (Human) 96Tests　025116

特徴

• Ficolin 1 (FCN1) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of FCN1 in human tissue homogenates, cell lysates and other biological fluids.
• Detection Range:0.156-10ng/ml
• Sensitivity:≤0.063ng/ml
• Intra-Assay:CV≤10%
• Inter-Assay:CV≤12%
• Kit Components:*025116A: Microtiter Plate, 1x96wells, Pre-coated, ready to use*025116B: Standard, 2x1vial 025116C: Standard Diluent, 1x20ml*025116D: Detection Reagent A, 1x120ul *025116E: Detection Reagent B, 1x120ul 025116F: Assay Diluent A, 1x12ml025116G: Assay Diluent B, 1x12ml025116H: TMB Substrate, 1x9ml025116K: Stop Solution, 1x6ml025116L: Wash Buffer, 30X, 1x20ml
• Precaution:The Stop Solution (025116K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
• Storage and Stability:Store *025116A, *025116B, *025116D and *025116E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Procedure Summary:1. Prepare all reagents, samples and standards
• 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C
• 3. Add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
• 4. Aspirate and wash 3 times
• 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
• 6. Aspirate and wash 5 times.