The Catalase (CAT) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CAT in human serum, plasma, cell lysates, tissue homogenates, cell culture supernatants and other biological fluids.
Detection Range:0.312-20ng/ml
Sensitivity:<0.113ng/ml
Precision:Intra-Assay: CV<10%Inter-Assay: CV<12%
Kit Components:*023986A: Microtiter Plate, 1x96 wells. Pre-coated; ready to use
Storage and Stability:Store *023986A, *023986B, *023986D and *023986E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate for 1 hour at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
