Platelet Derived Growth Factor Receptor Alpha (PDGFRa) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDGFRa in human tissue homogenates, cell lysates and other biological fluids.
Detection Range:0.156-50ng/ml
Sensitivity:0.060ng/ml
Intra-Assay:CV<10%
Inter-Assay:CV<12%
Kit Components:*027508A: Microtiter Plate, 1x96wells, Pre-coated, ready to use*027508B: Standard, 2x1vial 027508C: Standard Diluent, 1x20ml*027508D: Detection Reagent A (green), 1x120ul *027508E: Detection Reagent B (red), 1x120ul 027508F: Assay Diluent A, 2x, 1x6ml027508G: Assay Diluent B, 2x, 1x6ml027508H: TMB Substrate, 1x9ml027508K: Stop Solution, 1x6ml027508L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (027508K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *027508A, *027508B, *027508D and *027508E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap