Acrosin (ACR) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACR in human sperm.
Background:ACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.
Detection Range:0.156-10ng/ml
Sensitivity:0.062ng/ml
Kit Components:023089A: Microtiter Plate, 1x96 wells023089B: Standard (lyophilized), 2x1vial023089C: Detection Reagent A (100X), 1x120ul023089D: Detection Reagent B (100X), 1x120ul023089E: TMB Substrate, 1x9ml023089F: Wash Buffer (30X), 1x20ml023089G: Standard Diluent, 1x20ml023089H: Assay Diluent A (2X), 1x6ml023089J: Assay Diluent B (2X), 1x6ml023089K: Stop Solution, 1x6ml
Storage and Stability:Store 023089A, 023089B, 023089C, 023089D at -20°C upon receipt. Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
