Collagen Type I (COL1) BioAssay™ ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL1 in mouse serum, plasma and other biological fluids.
Detection Range:0.312-20ng/ml
Sensitivity:0.143ng/ml
Intra-Assay:CV<10%
Inter-Assay:CV<12%
Kit Components:*024260A: Microtiter Plate, 1x96wells, Pre-coated, ready to use*024260B: Standard, 2x1vial 024260C: Standard Diluent, 1x20ml*024260D: Detection Reagent A (green), 1x120ul *024260E: Detection Reagent B (red), 1x120ul 024260F: Assay Diluent A, 2x, 1x6ml024260G: Assay Diluent B, 2x, 1x6ml024260H: TMB Substrate, 1x9ml*024260J: Positive Control, 1x1vial024260K: Stop Solution, 1x6ml024260L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (024260K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *024260A, *024260B, *024260D, *024260E and *024260J at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
