Plasminogen Activator Inhibitor 1 (PAI1) BioAssay™ ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of PAI1 in rat plasma.
Detection Range:0.312-20ng/ml
Sensitivity:0.128ng/ml
Intra-Assay:CV < 10%
Inter-Assay:CV < 12%
Kit Components:*027472A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use*027472B: Standard, 2x1 vial 027472C: Standard Diluent, 1x20ml*027472D: Detection Reagent A (100X), 1x120ul *027472E: Detection Reagent B (100X), 1x120ul 027472F: Assay Diluent A (2X), 1x6ml027472G: Assay Diluent B (2X), 1x6ml027472H: TMB Substrate, 1x9ml*027472J: Positive Control, 37.54pg/ml, 1x1 vial027472K: Stop Solution, 1x6ml027472L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (027472K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *027472A, *027472B, *027472D and *027472E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.