Collagen Type III (COL3) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL3 in mouse serum, plasma, tissue homogenates and other biological fluids.
Detection Range:46.88-3000pg/ml
Sensitivity:18.24pg/ml
Intra-Assay:CV<10%
Inter-Assay:CV<12%
Kit Components:*024280A: Microtiter Plate, 1x48 wells or 1x96wells, Pre-coated, ready to use*024280B: Standard, 2x1vial 024280C: Standard Diluent, 1x10ml or 1x20ml*024280D: Detection Reagent A (green), 1x60ul or 1x120ul *024280E: Detection Reagent B (red), 1x60ul or 1x120ul 024280F: Assay Diluent A, 1x3ml or 1x6ml024280G: Assay Diluent B, 1x3ml or 1x6ml024280H: TMB Substrate, 1x4.5ml or 1x9ml*024280J: Positive Control, 755.6pg/ml, 1x1vial024280K: Stop Solution, 1x3ml or 1x6ml024280L: Wash Buffer, 30X, 1x10ml or 1x20ml
Precaution:The Stop Solution (024280K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *024280A, *024280B, *024280D, *024280E and *024280J at -20°C. After reconstitution of *024280J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 024280J: Positive Control:Intended Use:The control vial is from a mouse serum sample known to contain COL3. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics:The control vial contains mouse COL3 in mouse serum (lyophilized powder). Note: The mouse source material used in this product is from healthy mouse of SPF standards (specific pathogen-free animals).
Expected Values:755.6pg/ml
Usage:Dissolve with 150ul standard diluent and let it stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens
Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
