85-1735-73　Growth Hormone Releasing Hormone (GHRH) BioAssay™ ELISA Kit (Human) 96Tests　025511

特徴

• Growth Hormone Releasing Hormone (GHRH) BioAssay™ ELISA Kit (Human) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of GHRH in human serum, plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants and Other Biological Fluids.
• Detection Range:12.35-1000pg/ml
• Sensitivity:≤6.15pg/ml
• Intra-Assay:CV <10%
• Inter-Assay:CV <12%
• Kit Components:*025511A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use*025511B: Standard, 2x1vial 025511C: Standard Diluent, 1x20ml*025511D: Detection Reagent A, 1x120ul *025511E: Detection Reagent B, 1x120ul 025511F: Assay Diluent A, 2x, 1x6ml025511G: Assay Diluent B, 2x, 1x6ml025511H: TMB Substrate, 1x9ml025511K: Stop Solution, 1x6ml025511L: Wash Buffer, 30X, 1x20ml
• Precaution:The Stop Solution (025511K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
• Storage and Stability:Store *025511A, *025511B, *025511D and *025511E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Procedure Summary:1. Prepare all reagents, samples and standards
• 2. Add 50ul standard or sample to each well, then add 50ul working Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
• 3. Aspirate and wash 3 times
• 4. Add 100ul working Detection Reagent B. Incubate 30 minutes at 37°C
• 5. Aspirate and wash 5 times
• 6. Add 90ul 025511H: Substrate Solution. Incubate 15-25 minutes at 37°C
• 7. Add 50ul 025511K: Stop Solution. Read at 450nm immediately.