Monocyte Chemotactic Protein 1 (MCP1) BioAssay™ ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of MCP1 in rat serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Detection Range:0.156-10ng/ml
Sensitivity:0.061ng/ml
Intra-Assay:CV < 10%
Inter-Assay:CV < 12%
Kit Components:*026853A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use*026853B: Standard, 2x1 vial 026853C: Standard Diluent, 1x20ml*026853D: Detection Reagent A (100X), 1x120ul *026853E: Detection Reagent B (100X), 1x120ul 026853F: Assay Diluent A (2X), 1x6ml026853G: Assay Diluent B (2X), 1x6ml026853H: TMB Substrate, 1x9ml*026853J: Positive Control, 99.3pg/ml, 1x1 vial026853K: Stop Solution, 1x6ml026853L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (026853K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *026853A, *026853B, *026853D, *026853E and *026853J at -20°C. After reconstitution of *026853J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C
3. Add 100ul working Detection Reagent A immediately. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul working Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.
